ea hy926 immortalized human vascular endothelial cells  (ATCC)

Journal: RSC Advances

Article Title: Fabrication and characterization of electrospun polycaprolactone/ Urechis unicinctus derived-ECM composite scaffolds for small-diameter vascular grafts

doi: 10.1039/d5ra04406e

Figure Lengend Snippet: In vitro biocompatibility and cell behavior on pure PCL and PCL/UdECM composite scaffolds (1, 5, and 10 wt%). (a) SEM images at 12 h reveal cell morphology and attachment on scaffold surfaces. Scale bar = 30 μm. (b) Live/dead staining (green/red) images of EA.hy926 cells cultured on different scaffolds at day 1 and day 7, showing high cell viability and cell attachment across all groups. Scale bar = 100 μm. (c) Quantitative cell viability measured at Day 1 and 7 ( n = 4). (d) Cytoskeletal organization visualized by DAPI (nuclei, blue) and phalloidin (F-actin, green) staining at day 3 and day 14. Scale bar = 100 μm. (e) MTT assay results showing time-dependent cell proliferation on each scaffold ( n = 4). All values represent mean ± SD. Statistical analysis was performed using one-way ANOVA with Tukey's post hoc test. p < 0.05 (*), < 0.01 (**), and < 0.001 (***); NS = not significant.

Article Snippet: To evaluate cytocompatibility, EA.hy926 human vascular endothelial cells (ATCC, USA) were cultured in Dulbecco's Modified Eagle's Medium-High Glucose (DMEM-H, Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, USA) and 1% penicillin–streptomycin (P/S, Thermo Fisher Scientific, USA).

Techniques: In Vitro, Staining, Cell Culture, Cell Attachment Assay, MTT Assay

Journal: RSC Advances

Article Title: Fabrication and characterization of electrospun polycaprolactone/ Urechis unicinctus derived-ECM composite scaffolds for small-diameter vascular grafts

doi: 10.1039/d5ra04406e

Figure Lengend Snippet: Proangiogenic potential of PCL/UdECM composite scaffolds evaluated by in vitro tube formation assay. (a) Schematic illustration of the tube formation assay setup and the typical progression stages of endothelial tube formation: cell attachment, cluster arrangement, sprouting, and network maturation. EA.hy926 endothelial cells (5 × 10 4 cells per well) were seeded on Matrigel, and scaffolds were placed in Transwell inserts positioned above the cell layer. (b) Representative optical images of EA.hy926 cells cultured on different scaffolds (pure PCL and PCL/UdECM-1, -5, and -10) at 2 and 6 hours, showing enhanced tube formation with increasing UdECM content. Scale bar = 200 μm. (c–e) Quantitative analysis of angiogenic parameters at each time point: (c) number of junctions per field ( n = 3), (d) number of tubes per field ( n = 3), and (e) total tube length per field ( n = 3). All values represent mean ± SD. Statistical analysis was performed using one-way ANOVA with Tukey's post hoc test. p < 0.05 (*), < 0.01 (**), and < 0.001 (***); NS = not significant.

Article Snippet: To evaluate cytocompatibility, EA.hy926 human vascular endothelial cells (ATCC, USA) were cultured in Dulbecco's Modified Eagle's Medium-High Glucose (DMEM-H, Sigma-Aldrich, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, USA) and 1% penicillin–streptomycin (P/S, Thermo Fisher Scientific, USA).

Techniques: In Vitro, Tube Formation Assay, Cell Attachment Assay, Cell Culture